CellROX Green
CellROX Green (Ex-Max 485 nm/Em-Max 520 nm)
CellROX® Green is a fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ~ 485/520 nm. This reagent can be formaldehyde-fixed and its signal survives detergent treatment, which makes it able to be multiplexed with other compatible dyes and antibodies.
Excitation & emission spectra
Key facts
- Excitation maximum
- 485 nm
- Emission maximum
- 520 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
CellROX Green has an excitation maximum at 485 nm and an emission maximum at 520 nm, a Stokes shift of 35 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CellROX Green?
- CellROX® Green is a fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ~ 485/520 nm. This reagent can be formaldehyde-fixed and its signal survives detergent treatment, which makes it able to be multiplexed with other compatible dyes and antibodies.
- What laser excites CellROX Green?
- CellROX Green is optimally excited by the 488 laser line. Its excitation maximum is 485 nm.
- What filter should I use to detect CellROX Green?
- A 525/50 filter captures the peak of the CellROX Green emission near 520 nm with minimal spillover.