Alexa Fluor 647
Alexa Fluor™ 647 (Ex-Max 650 nm/Em-Max 668 nm)
Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 665 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 239000
- Quantum yield
- 0.33
- Molecular weight
- 800
- Commercial conjugates
- 4.7k from 27 suppliers
Alexa Fluor 647 has an excitation maximum at 650 nm and an emission maximum at 665 nm, a Stokes shift of 15 nm. Its extinction coefficient is 239000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Alexa Fluor 647?
- Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
- What laser excites Alexa Fluor 647?
- Alexa Fluor 647 is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect Alexa Fluor 647?
- A 660/20 filter captures the peak of the Alexa Fluor 647 emission near 665 nm with minimal spillover.
- How bright is Alexa Fluor 647?
- Alexa Fluor 647 has a relative brightness of 4 out of 5 (quantum yield 0.33).
- How many antibodies are available conjugated to Alexa Fluor 647?
- FluoroFinder tracks 4722 commercially available Alexa Fluor 647 conjugates, searchable by target, clone, host and application.