ECD
ECD (Ex-Max 595 nm/Em-Max 620 nm)
ECD is a tandem fluorophore designed for flow cytometry, spectrally similar to PE-CF®594, PE-Texas Red, PE-Dazzle 594 and PE-eFluor™ 610.
Excitation & emission spectra
Key facts
- Excitation maximum
- 595 nm
- Emission maximum
- 620 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 1960000
- Molecular weight
- 625000
ECD has an excitation maximum at 595 nm and an emission maximum at 620 nm, a Stokes shift of 25 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is ECD?
- ECD is a tandem fluorophore designed for flow cytometry, spectrally similar to PE-CF®594, PE-Texas Red, PE-Dazzle 594 and PE-eFluor™ 610.
- What laser excites ECD?
- ECD is optimally excited by the 488-561 laser line. Its excitation maximum is 595 nm.
- What filter should I use to detect ECD?
- A 610/20 filter captures the peak of the ECD emission near 620 nm with minimal spillover.
- How bright is ECD?
- ECD has a relative brightness of 3 out of 5.