GFP
GFP (Ex-Max 488 nm/Em-Max 510 nm)
Green Fluorescent Protein (GFP) is a weakly dimeric fluorescent protein isolated from Aequoria victoria and has excitation peaks at 395 and 475 nm and an emission peak at 509 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 510 nm
- Optimal laser line
- 488
- Optimal filter
- 510/50
- Extinction coefficient
- 56000
- Molecular weight
- 27000
GFP has an excitation maximum at 488 nm and an emission maximum at 510 nm, a Stokes shift of 22 nm. Its extinction coefficient is 56000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is GFP?
- Green Fluorescent Protein (GFP) is a weakly dimeric fluorescent protein isolated from Aequoria victoria and has excitation peaks at 395 and 475 nm and an emission peak at 509 nm.
- What laser excites GFP?
- GFP is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect GFP?
- A 510/50 filter captures the peak of the GFP emission near 510 nm with minimal spillover.