Emerald GFP
Emerald GFP (Ex-Max 487 nm/Em-Max 509 nm)
Emerald GFP is a monomeric, green-emitting fluorescent protein that has an excitation peak at 487 nm and an emission peak at 509 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 487 nm
- Emission maximum
- 509 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Extinction coefficient
- 57500
- Quantum yield
- 0.68
- Molecular weight
- 26900
Emerald GFP has an excitation maximum at 487 nm and an emission maximum at 509 nm, a Stokes shift of 22 nm. Its extinction coefficient is 57500 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Emerald GFP?
- Emerald GFP is a monomeric, green-emitting fluorescent protein that has an excitation peak at 487 nm and an emission peak at 509 nm.
- What laser excites Emerald GFP?
- Emerald GFP is optimally excited by the 488 laser line. Its excitation maximum is 487 nm.
- What filter should I use to detect Emerald GFP?
- A 515/30 filter captures the peak of the Emerald GFP emission near 509 nm with minimal spillover.