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MitoTracker Green

MitoTracker Green (Ex-Max 490 nm/Em-Max 509 nm)

MitoTracker Green from Thermo Fisher Scientific is a green fluorescent dye used to stain mitochondria in live cells.

Excitation & emission spectra

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Key facts

Excitation maximum
490 nm
Emission maximum
509 nm
Optimal laser line
488
Optimal filter
515/30

MitoTracker Green has an excitation maximum at 490 nm and an emission maximum at 509 nm, a Stokes shift of 19 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is MitoTracker Green?
MitoTracker Green from Thermo Fisher Scientific is a green fluorescent dye used to stain mitochondria in live cells.
What laser excites MitoTracker Green?
MitoTracker Green is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
What filter should I use to detect MitoTracker Green?
A 515/30 filter captures the peak of the MitoTracker Green emission near 509 nm with minimal spillover.