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Vybrant DyeCycle Green

Vybrant DyeCycle Green (Ex-Max 506 nm/Em-Max 534 nm)

Vybrant DyeCycle Green from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 506 nm and an emission peak at 534 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
506 nm
Emission maximum
534 nm
Optimal laser line
488
Optimal filter
525/50

Vybrant DyeCycle Green has an excitation maximum at 506 nm and an emission maximum at 534 nm, a Stokes shift of 28 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Vybrant DyeCycle Green?
Vybrant DyeCycle Green from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 506 nm and an emission peak at 534 nm.
What laser excites Vybrant DyeCycle Green?
Vybrant DyeCycle Green is optimally excited by the 488 laser line. Its excitation maximum is 506 nm.
What filter should I use to detect Vybrant DyeCycle Green?
A 525/50 filter captures the peak of the Vybrant DyeCycle Green emission near 534 nm with minimal spillover.