DyLight 488
DyLight™ 488 (Ex-Max 493 nm/Em-Max 518 nm)
DyLight™ 488 has an excitation peak at 493 nm and an emission peak at 518 nm and is spectrally similar to Alexa Fluor™ 488, fluorescein and FITC. DyLight™ 488 is most commonly used in flow cytometery, and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 493 nm
- Emission maximum
- 518 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 70000
- Commercial conjugates
- 1.4k from 23 suppliers
DyLight 488 has an excitation maximum at 493 nm and an emission maximum at 518 nm, a Stokes shift of 25 nm. Its extinction coefficient is 70000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DyLight 488?
- DyLight™ 488 has an excitation peak at 493 nm and an emission peak at 518 nm and is spectrally similar to Alexa Fluor™ 488, fluorescein and FITC. DyLight™ 488 is most commonly used in flow cytometery, and fluorescence microscopy applications.
- What laser excites DyLight 488?
- DyLight 488 is optimally excited by the 488 laser line. Its excitation maximum is 493 nm.
- What filter should I use to detect DyLight 488?
- A 525/50 filter captures the peak of the DyLight 488 emission near 518 nm with minimal spillover.
- How bright is DyLight 488?
- DyLight 488 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to DyLight 488?
- FluoroFinder tracks 1447 commercially available DyLight 488 conjugates, searchable by target, clone, host and application.