Spark Blue 574
Spark Blue™ 574 (Ex-Max 505 nm/Em-Max 570 nm)
Spark Blue™ 574 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 506 nm and an emission peak at 574 nm. This fluorophore is an alternative to Alexa Fluor™ 532 and can be efficiently excited by the 488 nm laser and can be unmixed from FITC and PE.
Excitation & emission spectra
Key facts
- Excitation maximum
- 505 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 488
- Optimal filter
- 585/42
- Relative brightness
- 1 of 5
- Commercial conjugates
- 21 from 2 suppliers
Spark Blue 574 has an excitation maximum at 505 nm and an emission maximum at 570 nm, a Stokes shift of 65 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Spark Blue 574?
- Spark Blue™ 574 from Biolegend is a fluorophore designed for spectral flow cytometry with an excitation peak at 506 nm and an emission peak at 574 nm. This fluorophore is an alternative to Alexa Fluor™ 532 and can be efficiently excited by the 488 nm laser and can be unmixed from FITC and PE.
- What laser excites Spark Blue 574?
- Spark Blue 574 is optimally excited by the 488 laser line. Its excitation maximum is 505 nm.
- What filter should I use to detect Spark Blue 574?
- A 585/42 filter captures the peak of the Spark Blue 574 emission near 570 nm with minimal spillover.
- How bright is Spark Blue 574?
- Spark Blue 574 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Spark Blue 574?
- FluoroFinder tracks 21 commercially available Spark Blue 574 conjugates, searchable by target, clone, host and application.