Mag-Fluo-4
Mag-Fluo-4 (Ex-Max 494 nm/Em-Max 516 nm)
Mag-Fluo-4 is a green fluorescent magnesium and calcium ion indicator that comes in various forms for different applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
Mag-Fluo-4 has an excitation maximum at 494 nm and an emission maximum at 516 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Mag-Fluo-4?
- Mag-Fluo-4 is a green fluorescent magnesium and calcium ion indicator that comes in various forms for different applications.
- What laser excites Mag-Fluo-4?
- Mag-Fluo-4 is optimally excited by the 488 laser line. Its excitation maximum is 494 nm.
- What filter should I use to detect Mag-Fluo-4?
- A 515/30 filter captures the peak of the Mag-Fluo-4 emission near 516 nm with minimal spillover.