Syto 16
Syto 16 (Ex-Max 488 nm/Em-Max 520 nm)
SYTO 16 from Thermo Fisher Scientific is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 494 nm and an emission peak at 525 nm. SYTO dyes can be used in flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 525 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
Syto 16 has an excitation maximum at 488 nm and an emission maximum at 525 nm, a Stokes shift of 37 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Syto 16?
- SYTO 16 from Thermo Fisher Scientific is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 494 nm and an emission peak at 525 nm. SYTO dyes can be used in flow cytometry and fluorescence microscopy.
- What laser excites Syto 16?
- Syto 16 is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect Syto 16?
- A 515/30 filter captures the peak of the Syto 16 emission near 525 nm with minimal spillover.