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iFluor 594

iFluor® 594 (Ex-Max 585 nm/Em-Max 606 nm)

iFluor® 594 is from AAT Bioquest is a fluorophore with an excitation peak at 585 nm and an emission peak at 606 nm. It can be useful in fluorescence microscopy. Spectrally, it is similar to Alexa Fluor™ 594 and Texas Red.

Excitation & emission spectra

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Key facts

Excitation maximum
585 nm
Emission maximum
606 nm
Optimal laser line
594
Optimal filter
610/20
Relative brightness
2 of 5
Commercial conjugates
9 from 3 suppliers

iFluor 594 has an excitation maximum at 585 nm and an emission maximum at 606 nm, a Stokes shift of 21 nm. It is optimally excited by the 594 laser line.

Frequently asked questions

What is iFluor 594?
iFluor® 594 is from AAT Bioquest is a fluorophore with an excitation peak at 585 nm and an emission peak at 606 nm. It can be useful in fluorescence microscopy. Spectrally, it is similar to Alexa Fluor™ 594 and Texas Red.
What laser excites iFluor 594?
iFluor 594 is optimally excited by the 594 laser line. Its excitation maximum is 585 nm.
What filter should I use to detect iFluor 594?
A 610/20 filter captures the peak of the iFluor 594 emission near 606 nm with minimal spillover.
How bright is iFluor 594?
iFluor 594 has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to iFluor 594?
FluoroFinder tracks 9 commercially available iFluor 594 conjugates, searchable by target, clone, host and application.