MitoLite Green FM
MitoLite Green FM (Ex-Max 490 nm/Em-Max 510 nm)
MitoLite Green FM from AAT Bioquest is a potential-independent green fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 490 nm and an emission peak at 510 nm. It can be used in fluorescence microscopy and flow cytometry and is not retained with fixation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 510 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
MitoLite Green FM has an excitation maximum at 490 nm and an emission maximum at 510 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is MitoLite Green FM?
- MitoLite Green FM from AAT Bioquest is a potential-independent green fluorescent dye used to stain mitochondria in live cells. It has an excitation max at 490 nm and an emission peak at 510 nm. It can be used in fluorescence microscopy and flow cytometry and is not retained with fixation.
- What laser excites MitoLite Green FM?
- MitoLite Green FM is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect MitoLite Green FM?
- A 515/30 filter captures the peak of the MitoLite Green FM emission near 510 nm with minimal spillover.