Fluo-3
Fluo-3 (Ex-Max 506 nm/Em-Max 527 nm)
Fluo-3, AM a cell-permeant calcium detection reagent. Fluo-3 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 506 nm and an emission peak at 527 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 506 nm
- Emission maximum
- 527 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Extinction coefficient
- 86000
- Molecular weight
- 855
Fluo-3 has an excitation maximum at 506 nm and an emission maximum at 527 nm, a Stokes shift of 21 nm. Its extinction coefficient is 86000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Fluo-3?
- Fluo-3, AM a cell-permeant calcium detection reagent. Fluo-3 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 506 nm and an emission peak at 527 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
- What laser excites Fluo-3?
- Fluo-3 is optimally excited by the 488 laser line. Its excitation maximum is 506 nm.
- What filter should I use to detect Fluo-3?
- A 525/50 filter captures the peak of the Fluo-3 emission near 527 nm with minimal spillover.