Acridine Orange
Acridine Orange (Ex-Max 500 nm/Em-Max 522 nm)
Acridine Orange is a fluorogenic cell-permeant nucleic acid stain that has an excitation peak at 500 nm and an emission peak at 582 nm. It can be useful in live or fixed cell cycle assays in flow cytometry and high content screening.
Excitation & emission spectra
Key facts
- Excitation maximum
- 500 nm
- Emission maximum
- 522 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
Acridine Orange has an excitation maximum at 500 nm and an emission maximum at 522 nm, a Stokes shift of 22 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Acridine Orange?
- Acridine Orange is a fluorogenic cell-permeant nucleic acid stain that has an excitation peak at 500 nm and an emission peak at 582 nm. It can be useful in live or fixed cell cycle assays in flow cytometry and high content screening.
- What laser excites Acridine Orange?
- Acridine Orange is optimally excited by the 488 laser line. Its excitation maximum is 500 nm.
- What filter should I use to detect Acridine Orange?
- A 525/50 filter captures the peak of the Acridine Orange emission near 522 nm with minimal spillover.