VRDye 490
VRDye 490 (Ex-Max 491 nm/Em-Max 515 nm)
VRDye 490 from LI-COR has an excitation peak at 491 nm and an emission peak at 515 nm. It is available in the form of secondary antibodies for fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 491 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
- Extinction coefficient
- 735000
- Molecular weight
- 1011
VRDye 490 has an excitation maximum at 491 nm and an emission maximum at 515 nm, a Stokes shift of 24 nm. Its extinction coefficient is 735000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is VRDye 490?
- VRDye 490 from LI-COR has an excitation peak at 491 nm and an emission peak at 515 nm. It is available in the form of secondary antibodies for fluorescence microscopy and flow cytometry.
- What laser excites VRDye 490?
- VRDye 490 is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
- What filter should I use to detect VRDye 490?
- A 515/30 filter captures the peak of the VRDye 490 emission near 515 nm with minimal spillover.
- How bright is VRDye 490?
- VRDye 490 has a relative brightness of 2 out of 5.