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violetFluor 500

VioletFluor 500 (Ex-Max 405 nm/Em-Max 500 nm)

VioletFluor® 500 is a fluorophore from Tonbo with an excitation peak at 405 nm and an emission peak at 500 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
405 nm
Emission maximum
500 nm
Optimal laser line
405
Optimal filter
525/50
Relative brightness
1 of 5
Commercial conjugates
8 from 2 suppliers

violetFluor 500 has an excitation maximum at 405 nm and an emission maximum at 500 nm, a Stokes shift of 95 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is violetFluor 500?
VioletFluor® 500 is a fluorophore from Tonbo with an excitation peak at 405 nm and an emission peak at 500 nm.
What laser excites violetFluor 500?
violetFluor 500 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
What filter should I use to detect violetFluor 500?
A 525/50 filter captures the peak of the violetFluor 500 emission near 500 nm with minimal spillover.
How bright is violetFluor 500?
violetFluor 500 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to violetFluor 500?
FluoroFinder tracks 8 commercially available violetFluor 500 conjugates, searchable by target, clone, host and application.