iFluor 555
iFluor® 555 (Ex-Max 556 nm/Em-Max 569 nm)
iFluor® 555 from AAT Bioquest is a fluorophore with an excitation peak at 556 nm and an emission peak at 559 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 555 and Cy3.
Excitation & emission spectra
Key facts
- Excitation maximum
- 556 nm
- Emission maximum
- 569 nm
- Optimal laser line
- 532
- Optimal filter
- 582/15
- Relative brightness
- 2 of 5
- Extinction coefficient
- 10000
- Quantum yield
- 0.64
- Molecular weight
- 1125.26
iFluor 555 has an excitation maximum at 556 nm and an emission maximum at 569 nm, a Stokes shift of 13 nm. Its extinction coefficient is 10000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is iFluor 555?
- iFluor® 555 from AAT Bioquest is a fluorophore with an excitation peak at 556 nm and an emission peak at 559 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 555 and Cy3.
- What laser excites iFluor 555?
- iFluor 555 is optimally excited by the 532 laser line. Its excitation maximum is 556 nm.
- What filter should I use to detect iFluor 555?
- A 582/15 filter captures the peak of the iFluor 555 emission near 569 nm with minimal spillover.
- How bright is iFluor 555?
- iFluor 555 has a relative brightness of 2 out of 5 (quantum yield 0.64).