Viafluor CFSE
Viafluor CFSE (Ex-Max 495 nm/Em-Max 515 nm)
Viafluor CFSE (CFDA-SE) is a fluorogenic, cell-permeant probe used in live-cell applications to assess cell health, for long term cell tracking or proliferation assays. CFDA-SE loads the cell passively but is retained once internalized by the subsequent esterase activity and conjugation of the succinimidyl ester moiety to intracellular amine-containing proteins and peptides.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
Viafluor CFSE has an excitation maximum at 495 nm and an emission maximum at 515 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Viafluor CFSE?
- Viafluor CFSE (CFDA-SE) is a fluorogenic, cell-permeant probe used in live-cell applications to assess cell health, for long term cell tracking or proliferation assays. CFDA-SE loads the cell passively but is retained once internalized by the subsequent esterase activity and conjugation of the succinimidyl ester moiety to intracellular amine-containing proteins and peptides.
- What laser excites Viafluor CFSE?
- Viafluor CFSE is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect Viafluor CFSE?
- A 515/30 filter captures the peak of the Viafluor CFSE emission near 515 nm with minimal spillover.