Live-or-Dye 488/515
Live-or-Dye™ 488/515 (Ex-Max 490 nm/Em-Max 516 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 488/515 has an excitation peak at 488 nm and an emission peak at 515 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 516 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial conjugates
- 2 from 1 supplier
Live-or-Dye 488/515 has an excitation maximum at 490 nm and an emission maximum at 516 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Live-or-Dye 488/515?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 488/515 has an excitation peak at 488 nm and an emission peak at 515 nm.
- What laser excites Live-or-Dye 488/515?
- Live-or-Dye 488/515 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect Live-or-Dye 488/515?
- A 515/30 filter captures the peak of the Live-or-Dye 488/515 emission near 516 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 488/515?
- FluoroFinder tracks 2 commercially available Live-or-Dye 488/515 conjugates, searchable by target, clone, host and application.