CF594
CF®594 (Ex-Max 593 nm/Em-Max 614 nm)
CF®594 from Biotium is a fluorophore with an excitation peak at 593 nm and an emission peak at 614 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 594, DyLight™ 594 and Texas Red. However, in flow cytometry, PE-CF®594 and PE-Dazzle 594 are superior alternatives. In microscopy, CF®594 has been validated in STED and 2-Photon microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 614 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 115000
- Molecular weight
- 730
- Commercial conjugates
- 3.7k from 2 suppliers
CF594 has an excitation maximum at 593 nm and an emission maximum at 614 nm, a Stokes shift of 21 nm. Its extinction coefficient is 115000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is CF594?
- CF®594 from Biotium is a fluorophore with an excitation peak at 593 nm and an emission peak at 614 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 594, DyLight™ 594 and Texas Red. However, in flow cytometry, PE-CF®594 and PE-Dazzle 594 are superior alternatives. In microscopy, CF®594 has been validated in STED and 2-Photon microscopy.
- What laser excites CF594?
- CF594 is optimally excited by the 594 laser line. Its excitation maximum is 593 nm.
- What filter should I use to detect CF594?
- A 610/20 filter captures the peak of the CF594 emission near 614 nm with minimal spillover.
- How bright is CF594?
- CF594 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to CF594?
- FluoroFinder tracks 3723 commercially available CF594 conjugates, searchable by target, clone, host and application.