CF488A
CF®488A (Ex-Max 490 nm/Em-Max 515 nm)
CF®488A from Biotium is a fluorophore with an excitation peak at 490 nm and an emission peak at 515 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry. In microscopy, CF®488A has been specifically validated for STORM, STED, Structured Illumination (SIM), 2-Photon microscopy and TIRF.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
- Extinction coefficient
- 70000
- Molecular weight
- 914
- Commercial conjugates
- 3.7k from 2 suppliers
CF488A has an excitation maximum at 490 nm and an emission maximum at 515 nm, a Stokes shift of 25 nm. Its extinction coefficient is 70000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CF488A?
- CF®488A from Biotium is a fluorophore with an excitation peak at 490 nm and an emission peak at 515 nm. It is spectrally similar to Fluorescein, Alexa Fluor™ 488 and DyLight™ 488. It can be use in fluorescence microscopy and flow cytometry. In microscopy, CF®488A has been specifically validated for STORM, STED, Structured Illumination (SIM), 2-Photon microscopy and TIRF.
- What laser excites CF488A?
- CF488A is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect CF488A?
- A 515/30 filter captures the peak of the CF488A emission near 515 nm with minimal spillover.
- How bright is CF488A?
- CF488A has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF488A?
- FluoroFinder tracks 3735 commercially available CF488A conjugates, searchable by target, clone, host and application.