VivaFix 408/512
VivaFix 408/512 (Ex-Max 408 nm/Em-Max 512 nm)
VivaFix 408/512 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 408 nm and an emission peak at 512 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 408 nm
- Emission maximum
- 512 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
VivaFix 408/512 has an excitation maximum at 408 nm and an emission maximum at 512 nm, a Stokes shift of 104 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is VivaFix 408/512?
- VivaFix 408/512 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 408 nm and an emission peak at 512 nm.
- What laser excites VivaFix 408/512?
- VivaFix 408/512 is optimally excited by the 405 laser line. Its excitation maximum is 408 nm.
- What filter should I use to detect VivaFix 408/512?
- A 515/30 filter captures the peak of the VivaFix 408/512 emission near 512 nm with minimal spillover.