What laser excites Brilliant Ultraviolet 661?

Brilliant Ultraviolet 661 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.

What filter should I use to detect Brilliant Ultraviolet 661?

A 660/20 filter captures the peak of the Brilliant Ultraviolet 661 emission near 661 nm with minimal spillover.

How bright is Brilliant Ultraviolet 661?

Brilliant Ultraviolet 661 has a relative brightness of 3 out of 5.

How many antibodies are available conjugated to Brilliant Ultraviolet 661?

FluoroFinder tracks 919 commercially available Brilliant Ultraviolet 661 conjugates, searchable by target, clone, host and application.

Brilliant Ultraviolet 661

BUV661 (Ex-Max 348 nm/Em-Max 661 nm)

Brilliant™ UltraViolet 661 (BUV661) is a red-emitting tandem fluorophore that combines BD Horizon® BUV395 and an acceptor dye. It can be excited by the 355 nm UV laser and collected using a 670/25 bandpass filter. BUV661 has an excitation peak at 498 nm and an emission peak at 657 nm This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.

Excitation & emission spectra

Key facts

Excitation maximum: 348 nm
Emission maximum: 661 nm
Optimal laser line: 355
Optimal filter: 660/20
Relative brightness: 3 of 5
Commercial antibodies: 919 from 2 suppliers

Brilliant Ultraviolet 661 has an excitation maximum at 348 nm and an emission maximum at 661 nm, a Stokes shift of 313 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Brilliant Ultraviolet 661?: Brilliant™ UltraViolet 661 (BUV661) is a red-emitting tandem fluorophore that combines BD Horizon® BUV395 and an acceptor dye. It can be excited by the 355 nm UV laser and collected using a 670/25 bandpass filter. BUV661 has an excitation peak at 498 nm and an emission peak at 657 nm This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.
What laser excites Brilliant Ultraviolet 661?: Brilliant Ultraviolet 661 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.
What filter should I use to detect Brilliant Ultraviolet 661?: A 660/20 filter captures the peak of the Brilliant Ultraviolet 661 emission near 661 nm with minimal spillover.
How bright is Brilliant Ultraviolet 661?: Brilliant Ultraviolet 661 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to Brilliant Ultraviolet 661?: FluoroFinder tracks 919 commercially available Brilliant Ultraviolet 661 conjugates, searchable by target, clone, host and application.