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EMA
EMA (Ex-Max 384 nm/Em-Max 593 nm)
Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. The fluorescence of ethidium monoazide is weak, but the intensity increases ∼15-fold on binding to DNA with excitation/emission maxima of ∼504/600 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 384 nm
- Emission maximum
- 593 nm
- Optimal laser line
- 405
- Optimal filter
- 585/42
- Commercial antibodies
- 1 from 1 supplier
EMA has an excitation maximum at 384 nm and an emission maximum at 593 nm, a Stokes shift of 209 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is EMA?
- Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. The fluorescence of ethidium monoazide is weak, but the intensity increases ∼15-fold on binding to DNA with excitation/emission maxima of ∼504/600 nm.
- What laser excites EMA?
- EMA is optimally excited by the 405 laser line. Its excitation maximum is 384 nm.
- What filter should I use to detect EMA?
- A 585/42 filter captures the peak of the EMA emission near 593 nm with minimal spillover.
- How many antibodies are available conjugated to EMA?
- FluoroFinder tracks 1 commercially available EMA conjugates, searchable by target, clone, host and application.