Fluo-4, AM a cell-permeant calcium detection reagent. Fluo-4 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 494 nm and an emission peak at 516 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.

What laser excites Fluo-4 AM?

Fluo-4 AM is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.

What filter should I use to detect Fluo-4 AM?

A 525/50 filter captures the peak of the Fluo-4 AM emission near 515 nm with minimal spillover.

How many antibodies are available conjugated to Fluo-4 AM?

FluoroFinder tracks 1 commercially available Fluo-4 AM conjugates, searchable by target, clone, host and application.

Fluo-4 AM Dye Profile | FluoroFinder

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Excitation & emission spectra

Key facts

Excitation maximum: 491 nm
Emission maximum: 515 nm
Optimal laser line: 488
Optimal filter: 525/50
Commercial antibodies: 1 from 1 supplier

Fluo-4 AM has an excitation maximum at 491 nm and an emission maximum at 515 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is Fluo-4 AM?: Fluo-4, AM a cell-permeant calcium detection reagent. Fluo-4 is the hydrolyzed reagent for microplate assays or as a standard control. It has an excitation peak at 494 nm and an emission peak at 516 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
What laser excites Fluo-4 AM?: Fluo-4 AM is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
What filter should I use to detect Fluo-4 AM?: A 525/50 filter captures the peak of the Fluo-4 AM emission near 515 nm with minimal spillover.
How many antibodies are available conjugated to Fluo-4 AM?: FluoroFinder tracks 1 commercially available Fluo-4 AM conjugates, searchable by target, clone, host and application.