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eGFP
eGFP (Ex-Max 488 nm/Em-Max 507 nm)
Enhanced GFP (eGFP) is a weakly dimeric, green-emitting fluorescent protein. It is a derivative of GFP, first isolated from the jellyfish Aequorea victoria. This variant was developed in 1995 to allow for the practical use of GFPs in mammalian cells. It has an excitation peak at 488 nm and an emission peak at 513 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 507 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Extinction coefficient
- 55900
- Quantum yield
- 0.6
- Molecular weight
- 26900
eGFP has an excitation maximum at 488 nm and an emission maximum at 507 nm, a Stokes shift of 19 nm. Its extinction coefficient is 55900 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is eGFP?
- Enhanced GFP (eGFP) is a weakly dimeric, green-emitting fluorescent protein. It is a derivative of GFP, first isolated from the jellyfish Aequorea victoria. This variant was developed in 1995 to allow for the practical use of GFPs in mammalian cells. It has an excitation peak at 488 nm and an emission peak at 513 nm.
- What laser excites eGFP?
- eGFP is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect eGFP?
- A 525/50 filter captures the peak of the eGFP emission near 507 nm with minimal spillover.