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PE-Cy5
PE-Cy5 (Ex-Max 496 nm/Em-Max 667 nm)
PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5. PE-Cy5 has an excitation peak at 496 nm and an emission peak at 667 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 496 nm
- Emission maximum
- 667 nm
- Optimal laser line
- 488-561
- Optimal filter
- 670/30
- Relative brightness
- 5 of 5
- Extinction coefficient
- 1960000
- Quantum yield
- 0.84
- Molecular weight
- 250000
- Commercial antibodies
- 2.4k from 29 suppliers
PE-Cy5 has an excitation maximum at 496 nm and an emission maximum at 667 nm, a Stokes shift of 171 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Cy5?
- PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5. PE-Cy5 has an excitation peak at 496 nm and an emission peak at 667 nm.
- What laser excites PE-Cy5?
- PE-Cy5 is optimally excited by the 488-561 laser line. Its excitation maximum is 496 nm.
- What filter should I use to detect PE-Cy5?
- A 670/30 filter captures the peak of the PE-Cy5 emission near 667 nm with minimal spillover.
- How bright is PE-Cy5?
- PE-Cy5 has a relative brightness of 5 out of 5 (quantum yield 0.84).
- How many antibodies are available conjugated to PE-Cy5?
- FluoroFinder tracks 2350 commercially available PE-Cy5 conjugates, searchable by target, clone, host and application.