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RealBlue 575
RB575
RealBlue 575 is a fluorescent dye produced by Waters Biosciences (formerly BD Biosciences) intended for use in spectral flow cytometry. It's excited by the blue laser (ex peak at 508 nm), and peak emission is 573 nm. Its moderate brightness makes it good for medium or high expression surface and intracellular markers.
Excitation & emission spectra
Key facts
- Excitation maximum
- 508 nm
- Emission maximum
- 573 nm
- Optimal laser line
- 488
- Relative brightness
- 2 of 5
- Commercial antibodies
- 586 from 1 supplier
RealBlue 575 has an excitation maximum at 508 nm and an emission maximum at 573 nm, a Stokes shift of 65 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RealBlue 575?
- RealBlue 575 is a fluorescent dye produced by Waters Biosciences (formerly BD Biosciences) intended for use in spectral flow cytometry. It's excited by the blue laser (ex peak at 508 nm), and peak emission is 573 nm. Its moderate brightness makes it good for medium or high expression surface and intracellular markers.
- What laser excites RealBlue 575?
- RealBlue 575 is optimally excited by the 488 laser line. Its excitation maximum is 508 nm.
- How bright is RealBlue 575?
- RealBlue 575 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to RealBlue 575?
- FluoroFinder tracks 586 commercially available RealBlue 575 conjugates, searchable by target, clone, host and application.