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NovaFluor Red 800

NF-R800

The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Red 800 has an excitation peak at 637 nm and an emission peak at 800 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
637 nm
Emission maximum
800 nm
Optimal laser line
633–640
Optimal filter
785LP
Relative brightness
3 of 5
Commercial antibodies
14 from 1 supplier

NovaFluor Red 800 has an excitation maximum at 637 nm and an emission maximum at 800 nm, a Stokes shift of 163 nm. It is optimally excited by the 633–640 laser line.

Frequently asked questions

What is NovaFluor Red 800?
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Red 800 has an excitation peak at 637 nm and an emission peak at 800 nm.
What laser excites NovaFluor Red 800?
NovaFluor Red 800 is optimally excited by the 633–640 laser line. Its excitation maximum is 637 nm.
What filter should I use to detect NovaFluor Red 800?
A 785LP filter captures the peak of the NovaFluor Red 800 emission near 800 nm with minimal spillover.
How bright is NovaFluor Red 800?
NovaFluor Red 800 has a relative brightness of 3 out of 5.
How many antibodies are available conjugated to NovaFluor Red 800?
FluoroFinder tracks 14 commercially available NovaFluor Red 800 conjugates, searchable by target, clone, host and application.