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Janelia Fluor 635b, NHS ester
Janelia Fluor 635b, NHS ester has an excitation peak at 654 nm and an emission peak at 671 nm. Janelia Fluor 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM). Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments. Derived from Janelia Fluor® 635, SE.
Excitation & emission spectra
Key facts
- Excitation maximum
- 654 nm
- Emission maximum
- 671 nm
- Molecular weight
- 615.71
Janelia Fluor 635b, NHS ester has an excitation maximum at 654 nm and an emission maximum at 671 nm, a Stokes shift of 17 nm.
Frequently asked questions
- What is Janelia Fluor 635b, NHS ester?
- Janelia Fluor 635b, NHS ester has an excitation peak at 654 nm and an emission peak at 671 nm. Janelia Fluor 635b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for DNA labelling using amine-terminated oligonucleotides. Also used to label oligonucleotides for super-resolution assays of transposase-accessible chromatin (ATAC-SMLM). Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments. Derived from Janelia Fluor® 635, SE.