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PhenoVue Cal-590 AM, Calcium Indicator

PhenoVue Cal-590 AM, Calcium Indicator (Ex-Max 581 nm/Em-Max 593 nm)

PhenoVue Cal-590 AM Bright is a red fluorescent calcium-sensitive dye which exhibits higher brightness, shorter incubation time, improved signal-to-noise ratio, reduced leakage, and higher intracellular retention, as well as homogeneous staining compared to other red calcium indicators such as Rhod-2 and PhenoVue Cal-590 AM. PhenoVue Cal-590 AM Bright is ideal for multiplexing experiments including FITC, PhenoVue Fluor 488 or GFP.

Excitation & emission spectra

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Key facts

Excitation maximum
581 nm
Emission maximum
593 nm
Optimal laser line
561

PhenoVue Cal-590 AM, Calcium Indicator has an excitation maximum at 581 nm and an emission maximum at 593 nm, a Stokes shift of 12 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is PhenoVue Cal-590 AM, Calcium Indicator?
PhenoVue Cal-590 AM Bright is a red fluorescent calcium-sensitive dye which exhibits higher brightness, shorter incubation time, improved signal-to-noise ratio, reduced leakage, and higher intracellular retention, as well as homogeneous staining compared to other red calcium indicators such as Rhod-2 and PhenoVue Cal-590 AM. PhenoVue Cal-590 AM Bright is ideal for multiplexing experiments including FITC, PhenoVue Fluor 488 or GFP.
What laser excites PhenoVue Cal-590 AM, Calcium Indicator?
PhenoVue Cal-590 AM, Calcium Indicator is optimally excited by the 561 laser line. Its excitation maximum is 581 nm.