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LysoBrite Blue
LysoBrite Blue (Ex-Max 432 nm/Em-Max 480 nm)
LysoBrite Blue from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Blue has an excitation peak at 432 nm and an emission peak at 480 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 432 nm
- Emission maximum
- 480 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
LysoBrite Blue has an excitation maximum at 432 nm and an emission maximum at 480 nm, a Stokes shift of 48 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is LysoBrite Blue?
- LysoBrite Blue from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Blue has an excitation peak at 432 nm and an emission peak at 480 nm.
- What laser excites LysoBrite Blue?
- LysoBrite Blue is optimally excited by the 405 laser line. Its excitation maximum is 432 nm.
- What filter should I use to detect LysoBrite Blue?
- A 450/50 filter captures the peak of the LysoBrite Blue emission near 480 nm with minimal spillover.