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BODIPY 493/503

BODIPY 493/503 (Ex-Max 490 nm/Em-Max 501 nm)

BODIPY 493/503 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 493 nm and an emission peak at 503 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.

Excitation & emission spectra

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Key facts

Excitation maximum
490 nm
Emission maximum
501 nm
Optimal laser line
488
Optimal filter
515/30
Molecular weight
262.1085
Commercial antibodies
1 from 1 supplier

BODIPY 493/503 has an excitation maximum at 490 nm and an emission maximum at 501 nm, a Stokes shift of 11 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is BODIPY 493/503?
BODIPY 493/503 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 493 nm and an emission peak at 503 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
What laser excites BODIPY 493/503?
BODIPY 493/503 is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
What filter should I use to detect BODIPY 493/503?
A 515/30 filter captures the peak of the BODIPY 493/503 emission near 501 nm with minimal spillover.
How many antibodies are available conjugated to BODIPY 493/503?
FluoroFinder tracks 1 commercially available BODIPY 493/503 conjugates, searchable by target, clone, host and application.