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MitoView 405
MitoView 405 (Ex-Max 398 nm/Em-Max 440 nm)
Biotium's MitoView dyes are used for live cell mitochondrial staining. These dyes are bright, photostable, non-toxic dyes, and do not require a wash step before Fluorescence Microscopy. While these dyes should be used for live cell staining only, the exception is MitoView Green which can be used for staining fixed cells. MitoView 405 has an excitation peak at 398 nm and an emission peak at 440 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 398 nm
- Emission maximum
- 440 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 1 supplier
MitoView 405 has an excitation maximum at 398 nm and an emission maximum at 440 nm, a Stokes shift of 42 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is MitoView 405?
- Biotium's MitoView dyes are used for live cell mitochondrial staining. These dyes are bright, photostable, non-toxic dyes, and do not require a wash step before Fluorescence Microscopy. While these dyes should be used for live cell staining only, the exception is MitoView Green which can be used for staining fixed cells. MitoView 405 has an excitation peak at 398 nm and an emission peak at 440 nm.
- What laser excites MitoView 405?
- MitoView 405 is optimally excited by the 405 laser line. Its excitation maximum is 398 nm.
- What filter should I use to detect MitoView 405?
- A 450/50 filter captures the peak of the MitoView 405 emission near 440 nm with minimal spillover.
- How many antibodies are available conjugated to MitoView 405?
- FluoroFinder tracks 2 commercially available MitoView 405 conjugates, searchable by target, clone, host and application.