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NucSpot Live 488
NucSpot Live 488 (Ex-Max 503 nm/Em-Max 518 nm)
Biotium's NucSpot Live 488 is a cell membrane-permeant dye that can passively enter live cells to assess DNA content analysis or counterstain nuclei in flow cytometry and fluorescence microscopy applications. It has an excitation peak at 503 nm and an emission peak at 518 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 503 nm
- Emission maximum
- 518 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial antibodies
- 2 from 1 supplier
NucSpot Live 488 has an excitation maximum at 503 nm and an emission maximum at 518 nm, a Stokes shift of 15 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NucSpot Live 488?
- Biotium's NucSpot Live 488 is a cell membrane-permeant dye that can passively enter live cells to assess DNA content analysis or counterstain nuclei in flow cytometry and fluorescence microscopy applications. It has an excitation peak at 503 nm and an emission peak at 518 nm.
- What laser excites NucSpot Live 488?
- NucSpot Live 488 is optimally excited by the 488 laser line. Its excitation maximum is 503 nm.
- What filter should I use to detect NucSpot Live 488?
- A 515/30 filter captures the peak of the NucSpot Live 488 emission near 518 nm with minimal spillover.
- How many antibodies are available conjugated to NucSpot Live 488?
- FluoroFinder tracks 2 commercially available NucSpot Live 488 conjugates, searchable by target, clone, host and application.