Antibody Search
OMIPs
eLearning 
Dye Directory
alamarBlue
alamarBlue (Ex-Max 569 nm/Em-Max 582 nm)
alamarBlue (Resazurin) is a cell-permeant fluorogenic probe that passively enters a healthy cell where it is reduced to a brightly emitting dye called Resarufin. It can be used in fluorescent or colorimetic microplate assays and is useful to detect the health of animal cell lines, bacteria, plant and fungi. Fluorescently it is excited at 569 nm and emits at 582 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 569 nm
- Emission maximum
- 582 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
alamarBlue has an excitation maximum at 569 nm and an emission maximum at 582 nm, a Stokes shift of 13 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is alamarBlue?
- alamarBlue (Resazurin) is a cell-permeant fluorogenic probe that passively enters a healthy cell where it is reduced to a brightly emitting dye called Resarufin. It can be used in fluorescent or colorimetic microplate assays and is useful to detect the health of animal cell lines, bacteria, plant and fungi. Fluorescently it is excited at 569 nm and emits at 582 nm.
- What laser excites alamarBlue?
- alamarBlue is optimally excited by the 561 laser line. Its excitation maximum is 569 nm.
- What filter should I use to detect alamarBlue?
- A 585/42 filter captures the peak of the alamarBlue emission near 582 nm with minimal spillover.