Loading the Dye Directory…

ATTO 390

ATTO 390 (Ex-Max 390 nm/Em-Max 479 nm)

ATTO 390 from ATTO-TEC has an excitation peak at 390 nm and an emission peak at 448 nm. It is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, good photo-stability, and low molecular weight.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
390 nm
Emission maximum
479 nm
Optimal laser line
405
Optimal filter
450/50
Relative brightness
3 of 5
Extinction coefficient
24000
Quantum yield
0.9
Fluorescence lifetime
5.0 ns
Molecular weight
368
Commercial antibodies
1.7k from 8 suppliers

ATTO 390 has an excitation maximum at 390 nm and an emission maximum at 479 nm, a Stokes shift of 89 nm. Its extinction coefficient is 24000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.

Frequently asked questions

What is ATTO 390?
ATTO 390 from ATTO-TEC has an excitation peak at 390 nm and an emission peak at 448 nm. It is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, good photo-stability, and low molecular weight.
What laser excites ATTO 390?
ATTO 390 is optimally excited by the 405 laser line. Its excitation maximum is 390 nm.
What filter should I use to detect ATTO 390?
A 450/50 filter captures the peak of the ATTO 390 emission near 479 nm with minimal spillover.
How bright is ATTO 390?
ATTO 390 has a relative brightness of 3 out of 5 (quantum yield 0.9).
How many antibodies are available conjugated to ATTO 390?
FluoroFinder tracks 1677 commercially available ATTO 390 conjugates, searchable by target, clone, host and application.