IRDye® 800CW is excited by the laser and emission captured by the 780/60 filter. It can be used with the following analysis platforms: Fluorescence Microscopy, Western Blot, Immunohistochemistry.

What laser excites IRDye 800CW?

IRDye 800CW is optimally excited by the 750 laser line. Its excitation maximum is 774 nm.

What filter should I use to detect IRDye 800CW?

A 780/60 filter captures the peak of the IRDye 800CW emission near 789 nm with minimal spillover.

How bright is IRDye 800CW?

IRDye 800CW has a relative brightness of 1 out of 5.

How many antibodies are available conjugated to IRDye 800CW?

FluoroFinder tracks 27 commercially available IRDye 800CW conjugates, searchable by target, clone, host and application.

IRDye 800CW Dye Profile

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Excitation & emission spectra

Key facts

Excitation maximum: 774 nm
Emission maximum: 789 nm
Optimal laser line: 750
Optimal filter: 780/60
Relative brightness: 1 of 5
Commercial antibodies: 27 from 2 suppliers

IRDye 800CW has an excitation maximum at 774 nm and an emission maximum at 789 nm, a Stokes shift of 15 nm. It is optimally excited by the 750 laser line.

Frequently asked questions

What is IRDye 800CW?: IRDye® 800CW is excited by the laser and emission captured by the 780/60 filter. It can be used with the following analysis platforms: Fluorescence Microscopy, Western Blot, Immunohistochemistry.
What laser excites IRDye 800CW?: IRDye 800CW is optimally excited by the 750 laser line. Its excitation maximum is 774 nm.
What filter should I use to detect IRDye 800CW?: A 780/60 filter captures the peak of the IRDye 800CW emission near 789 nm with minimal spillover.
How bright is IRDye 800CW?: IRDye 800CW has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to IRDye 800CW?: FluoroFinder tracks 27 commercially available IRDye 800CW conjugates, searchable by target, clone, host and application.