What laser excites Live-or-Dye 405/452?

Live-or-Dye 405/452 is optimally excited by the 405 laser line. Its excitation maximum is 416 nm.

What filter should I use to detect Live-or-Dye 405/452?

A 450/50 filter captures the peak of the Live-or-Dye 405/452 emission near 452 nm with minimal spillover.

How many antibodies are available conjugated to Live-or-Dye 405/452?

FluoroFinder tracks 2 commercially available Live-or-Dye 405/452 conjugates, searchable by target, clone, host and application.

Loading the Dye Directory…

Live-or-Dye 405/452

Live-or-Dye™ 405/452 (Ex-Max 416 nm/Em-Max 452 nm)

Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 405/452 has an excitation peak at 405 nm and an emission peak at 452 nm.

Excitation & emission spectra

Key facts

Excitation maximum: 416 nm
Emission maximum: 452 nm
Optimal laser line: 405
Optimal filter: 450/50
Commercial antibodies: 2 from 1 supplier

Live-or-Dye 405/452 has an excitation maximum at 416 nm and an emission maximum at 452 nm, a Stokes shift of 36 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Live-or-Dye 405/452?: Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 405/452 has an excitation peak at 405 nm and an emission peak at 452 nm.
What laser excites Live-or-Dye 405/452?: Live-or-Dye 405/452 is optimally excited by the 405 laser line. Its excitation maximum is 416 nm.
What filter should I use to detect Live-or-Dye 405/452?: A 450/50 filter captures the peak of the Live-or-Dye 405/452 emission near 452 nm with minimal spillover.
How many antibodies are available conjugated to Live-or-Dye 405/452?: FluoroFinder tracks 2 commercially available Live-or-Dye 405/452 conjugates, searchable by target, clone, host and application.