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CF680
CF®680 (Ex-Max 681 nm/Em-Max 698 nm)
CF®680 from Biotium is a fluorophore with an excitation peak at 681 nm and an emission peak at 698 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 681 nm
- Emission maximum
- 698 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 210000
- Molecular weight
- 3241
- Commercial antibodies
- 45 from 1 supplier
CF680 has an excitation maximum at 681 nm and an emission maximum at 698 nm, a Stokes shift of 17 nm. Its extinction coefficient is 210000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF680?
- CF®680 from Biotium is a fluorophore with an excitation peak at 681 nm and an emission peak at 698 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
- What laser excites CF680?
- CF680 is optimally excited by the 628-640 laser line. Its excitation maximum is 681 nm.
- What filter should I use to detect CF680?
- A 695/40 filter captures the peak of the CF680 emission near 698 nm with minimal spillover.
- How bright is CF680?
- CF680 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF680?
- FluoroFinder tracks 45 commercially available CF680 conjugates, searchable by target, clone, host and application.