What laser excites Alexa Fluor 750?

Alexa Fluor 750 is optimally excited by the 730-750 laser line. Its excitation maximum is 749 nm.

What filter should I use to detect Alexa Fluor 750?

A 780/60 filter captures the peak of the Alexa Fluor 750 emission near 775 nm with minimal spillover.

How bright is Alexa Fluor 750?

Alexa Fluor 750 has a relative brightness of 1 out of 5.

How many antibodies are available conjugated to Alexa Fluor 750?

FluoroFinder tracks 31064 commercially available Alexa Fluor 750 conjugates, searchable by target, clone, host and application.

Alexa Fluor 750

Alexa Fluor™ 750 (Ex-Max 749 nm/Em-Max 775 nm)

Alexa Fluor™ 750 (AF750, Alexa 750) has an excitation peak at 749 nm and an emission peak at 775 nm, and is spetrally similar to Cy®7 (GE Healthcare), APC-Fire™ 750 (BioLegend), CF®®750 (Biotium), DyLight™ 750 (ThermoFisher Scientific), iFluor® 750 (ATT Bioquest) and iFluor® 790 (ATT Bioquest). Alexa 750 is often used as a tandem fluorophore in flow cytometry or used on its own in fluorescence microscopy, in-vivo fluorescence microscopy or super-resolution microscopy applications.

Excitation & emission spectra

Key facts

Excitation maximum: 749 nm
Emission maximum: 775 nm
Optimal laser line: 730-750
Optimal filter: 780/60
Relative brightness: 1 of 5
Extinction coefficient: 240000
Commercial antibodies: 31.1k from 12 suppliers

Alexa Fluor 750 has an excitation maximum at 749 nm and an emission maximum at 775 nm, a Stokes shift of 26 nm. Its extinction coefficient is 240000 M⁻¹cm⁻¹. It is optimally excited by the 730-750 laser line.

Frequently asked questions

What is Alexa Fluor 750?: Alexa Fluor™ 750 (AF750, Alexa 750) has an excitation peak at 749 nm and an emission peak at 775 nm, and is spetrally similar to Cy®7 (GE Healthcare), APC-Fire™ 750 (BioLegend), CF®®750 (Biotium), DyLight™ 750 (ThermoFisher Scientific), iFluor® 750 (ATT Bioquest) and iFluor® 790 (ATT Bioquest). Alexa 750 is often used as a tandem fluorophore in flow cytometry or used on its own in fluorescence microscopy, in-vivo fluorescence microscopy or super-resolution microscopy applications.
What laser excites Alexa Fluor 750?: Alexa Fluor 750 is optimally excited by the 730-750 laser line. Its excitation maximum is 749 nm.
What filter should I use to detect Alexa Fluor 750?: A 780/60 filter captures the peak of the Alexa Fluor 750 emission near 775 nm with minimal spillover.
How bright is Alexa Fluor 750?: Alexa Fluor 750 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Alexa Fluor 750?: FluoroFinder tracks 31064 commercially available Alexa Fluor 750 conjugates, searchable by target, clone, host and application.